The study involved the evaluation of RNA samples from banked tumors from 74 Dukes’ B colon cancer patients. Each sample was analyzed using several extremely parallel methods for analysis of gene expression. Thirty-one of these sufferers developed tumor relapse in under 3 years, while 43 sufferers remained disease-free for more than three years after surgery. Armed with this information, two approaches were used to identify the genetic markers that can best discriminate between these two groups of patients, reaching the last 23-gene combination ultimately.‘This study shows that many Americans have had direct encounter using prescription discomfort relievers and a big share possess misused or abused these medications themselves, or have close friends or family members who have done so,’ study innovator Colleen Barry, an associate professor at Johns Hopkins Bloomberg School of Public Health, said in a university news release. Simultaneously, however, the researchers found that nearly 60 % of Americans believe abuse of the medications is a substantial general public health concern. That’s very similar to Americans’ views on other open public health concerns such as gun violence and tobacco use, the researchers said. In 2012, medication overdoses, involving prescription painkillers primarily, surpassed car crashes to become the leading reason behind injury death, the researchers said.